Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM161

$198.00 – $441.00

Rabbit monoclonal to Acetylated Histone H3 Lysine 9 (K9ac), H3K9ac, Histone H3 (K9 acetylation)

25 µg Trial Size, 100 µg Full Size

Tags:31-1054-00, 31-1054-00-S, 31105400, 31105400S
SKU:31-1054-00
Categories:Histone H3 Acetylation, Histone Modification, Primary Antibodies, Rabbit Monoclonal

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Description

Product Details
Product Name	Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM161
Product Description	Rabbit monoclonal to Acetylated Histone H3 Lysine 9 (K9ac), H3K9ac, Histone H3 (K9 acetylation)
Catalog No.	31-1054-00
Clone Name	RM161
Product Data Sheet	Anti-Acetyl-Histone H3 Lys9 Rabbit Monoclonal Antibody Data Sheet RM161
Specificity	RM161 reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3.
Immunogen	An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys9).
Application	Western Blot (WB) Chromatin IP (ChIP) Immunocytochemistry (ICC) Immunohistochemistry (IHC) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
.
Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 µg
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.25 µg/mL - 1 µg/mL; ICC:0.5 µg/mL - 2 µg/mL; ChIP: 2 µg/mL - 10 µg/mL; IHC: 0.1 µg/mL – 1 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.05 µg/mL – 0.5 µg/mL.
Keywords
References for Acetyl-Histone H3 (Lys9) antibody [RM161]	Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794) Bulut-Karslioglu A et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output. Cell Stem Cell. (2018) 10.1016/j.stem.2018.02.004. WB; Human. Read more (PubMed: 29499153)
Product Images
	RM161 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetyl- ated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
	Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM161 at 0.25 µg/mL, showed a band of histone H3 acetylated at Lysine 9 in treated HeLa.
	Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 µg/mL) as the capture antibody and biotinylated anti-H3K9ac (RM161, 1 µg/mL) as the detection antibody.
	ChIP performed on HeLa cells using H3K9ac antibody (RM161, 5 µg). Real-time PCR was performed using primers specific to the gene indicated.
	Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys9) Rabbit mAb RM161 (red).Actin filaments have been labeled with fluorescein phalloidin (green).
	Immunohistochemistry staining of HepG2 cells using anti-Acetyl-Histone H3 (Lys9) antibody, Clone RM161.