Anti-cAMP Rabbit Monoclonal Antibody, Clone RM466

Anti-cAMP Rabbit Monoclonal Antibody, Clone RM466

$385.00

Rabbit monoclonal to cAMP (Cyclic Adenosine Monophosphate)

Related Product: Catalog# RM-PA466 (71-1358-00), cAMP-HRP

50 µg


  • Tags:31-1358-00, 31135800
  • SKU:31-1358-00
  • Categories:ELISA, Primary Antibodies, Rabbit Monoclonal, Small Molecule
RevMAb in China:

Description

Product Details
Product NameAnti-cAMP Rabbit Monoclonal Antibody, Clone M466
Product DescriptionRabbit monoclonal to cAMP (Cyclic Adenosine Monophosphate)
Catalog No.31-1358-00
Clone NameRM466
Product Data SheetAnti-cAMP Rabbit Monoclonal Antibody Data Sheet RM466
SpecificityRM466 reacts to cAMP (Cyclic Adenosine Monophosphate). No cross reactivity with other cyclic nucleotide or nucleoside phosphate.
ImmunogenKLH-conjugated cAMP
ApplicationELISA
ConjugateNone
Intended UseResearch Use Only
Related Product Antigen for Competitive Assay RM-PA466 (71-1358-00) - cAMP-HRP
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size50 µg
Concentration 1 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.05 µg/mL – 0.5 µg/mL
Keywords
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Product Images

Competitive ELISA of cAMP using anti-cAMP (RM466). The 96-well plate was coated
with 1 µg/mL of Goat anti-rabbit IgG (50 µL/well). 0.05 µg/mL of anti-cAMP (RM466)
(50 µL/well) was added and incubated. After wash and block, different concentrations of cAMP
(25 µL/well) were added along with 25 µL/well of 1/5,000 diluted HRP conjugated cAMP.
(RM-PA466 (71-1358-00)). TMB was used to develop the color after incubation and wash.

Competitive ELISA showing the specificity of anti-cAMP (RM466). The 96-well
plate was coated with 1 µg/mL of Goat anti-rabbit IgG (50 µL/well). 0.05 µg/mL
of anti-cAMP (RM466) (50 µL/well) was added and incubated. After wash
and block, cAMP and other cyclic nucleotides or nucleoside phosphates (25 µL/well)
were added along with 25 µL/well of 1/5,000 diluted HRP conjugated cAMP
(RM-PA466 (71-1358-00)). TMB was used to develop the color after incubation and wash.

Competitive ELISA detecting cAMP in HeLa cells. The 96-well plate was coated with
1 µg/mL of Goat anti-rabbit IgG (50 µL/well). 0.05 µg/mL of anti-cAMP (RM466)
(50 µL/well) was added and incubated. After wash and block, HeLa cell lysate
(~105cell/mL) or control (lysate buffer only) (25 µL/well) were added along with
25 µL/well of 1/5,000 diluted HRP conjugated cAMP (RM-PA466 (71-1358-00)).
TMB was used to develop the color after incubation and wash.

RevMAb in China: AREX Biosciences Ltd. 400-886-0853 www.arexbio.com info@arexbio.com
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