China
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Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM140
$198.00 – $441.00
RevMAb in China:
| Product Details | |
| Product Name | Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM140 |
| Product Description | Rabbit monoclonal to Monomethylated Histone H3 Lysine 4, H3K4me1, Histone H3 (monomethyl K4) |
| Catalog No. | 31-1046-00 |
| Clone Name | RM140 |
| Product Data Sheet | Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM140 |
| Specificity | RM140 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3 |
| Immunogen | A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4) |
| Application | Western Blot (WB) Chromatin IP (ChIP) Immunocytochemistry (ICC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Properties | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.2 µg/mL – 1 µg/mL; ChIP: 2 µg/mL- 10 µg/mL; ICC: 1 µg/mL - 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 0.5 µg/mL. |
| Keywords | |
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| References | Shah RN et al Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies. Mol Cell. (2018), 10.1016/j.molcel.2018.08.015. ICeChIP. Read more (PubMed: 30244833) |
| Product Images | |
 | RM140 specifically reacts to Histone H3 monomethylated at |
 | Western Blot of recombinant histone H3.3 (1) and acid extracts |
 | ChIP performed on HeLa cells using H3K4me1 antibody |
 | Immunocytochemistry of HeLa cells treated with sodium butyrate, |
