Anti-His-Tag Chimeric Human Monoclonal Antibody, Clone RMH01

$386.00

Rabbit/Human Chimeric monoclonal to His-Tag (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)
containing Human IgG1 CH and Cκ

100 µg


  • Tags:54-1161-00, 54116100
  • SKU:54-1161-00
  • Categories:Epitope Tags, Primary Antibodies, Rabbit/Human Chimeric Antibody
RevMAb in China:

Description

Product Details
Product NameAnti-His-Tag Rabbit/Human Chimeric Monoclonal Antibody, Clone RMH01
Product DescriptionRabbit/Human Chimeric monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)

This antibody is a Rabbit-Human Chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.
Catalog No.54-1161-00
Clone NameRMH01
Product Data SheetAnti-Rabbit/Human Chimeric His-Tag Monoclonal Antibody Data Sheet RMH01
SpecificityRMH01 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein in mammalian or bacteria cells.
ImmunogenMixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
ApplicationWestern Blot
ELISA (Capturer or Detector)
Species ReactivityAll
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 µg
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit/Human Chimeric
Rabbit Fv/ Human IgG CH and Cκ
UsageELISA: Capturer: 0.1 µg/mL – 2 µg/mL;
Detector: 0.01 µg/mL – 0.5 µg/mL;
Western Blot: 0.1 µg/mL – 1 µg/mL.
Keywords
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Product Images
Sandwich

Sandwich ELISA performed on different amounts of
whole lysate of 293T cells transfected with a DNA
construct encoding HisTag Histone H3 (K36M) protein,
using 50 µL/well of different concentrations of chimeric
human anti-HisTag antibody, Clone RMH01, as the
capture antibody. Anti-H3K36M rabbit monoclonal
antibody (RM193) was used as the detection antibody,
followed by an alkaline phosphatase conjugated Rabbit
IgG as the Secondary antibody.

A

A titer ELISA performed on purified HisTag-PCSK9
recombinant protein. A serial dilution of chimeric
human anti-HisTag antibody, Clone RMH01, was used
as the primary antibody. An alkaline phosphatase
conjugated anti-Human IgG as the secondary antibody.

Western

Western blot of 293T cells transfected (+) or untransfected (-)
with a DNA construct encoding His-Tag Histone H3 (G34W)
protein, using chimeric human anti-HisTag antibody, Clone RMH01,
at 0.2ug/mL, followed by a HRP conjugated anti-Human IgG
secondary antibody.

RevMAb in China: AREX Biosciences Ltd. 400-886-0853 www.arexbio.com info@arexbio.com

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