Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM340; ChIP Validated

$198.00 – $441.00

Rabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4)

25 µg Trial Size, 100 µg Full Size


  • Tags:31-1226-00, 31-1226-00-S, 31122600, 31122600S
  • SKU:31-1226-00
  • Categories:Histone H3 Methylation, Histone Modification, Primary Antibodies, Rabbit Monoclonal
RevMAb in China:

Description

Product Details
Product NameAnti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM340
Product DescriptionRabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4)
Catalog No.31-1226-00
Clone NameRM340
Product Data SheetAnti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM340
SpecificityRM340 reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1), dimethylated Lysine 4 (K4me3), or other methylations in histone H3.
ImmunogenA trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4).
ApplicationChromatin IP (ChIP)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 µg
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
Usage"ChIP: 1 µg/mL – 5 µg/mL;
ELISA: 1 µg/mL – 10 µg/mL;
Multiplex: 0.5 µg/mL – 2 µg/mL.
Keywords
.
Product Images
SNAP-ChIP

SNAP-ChIP TM / qPCR using anti-H3K4me3 rabbit
monoclonal antibody, Clone RM340. Antibody (3 μg at
17 μg/mL) was tested in native ChIP with 3 μg HEK-
293 chromatin (~1x106 cells). Specificity (left Y-axis;
all bars mean ± SEM from six independent ChIP
experiments; noteH3K4me3) was determined by quantitative real-time
PCR (qPCR) for the duplicate DNA barcodes
corresponding to each modified nucleosome in the
SNAP-ChIP K-MetStat panel (X-axis). Black bar
represents antibody efficiency (right Y-axis; log scale)
and indicates percentage of the barcoded H3K4me3
nucleosome target immunoprecipitated relative to Input.

ELISA



ELISA of Designer Nucleosomes (dNucs)
(Recombinant Human Nucleosome with H3 K4
Modified), using anti-H3K4me3 rabbit monoclonal
antibody, Clone RM340 (10 μg/mL).

RevMAb in China: AREX Biosciences Ltd. 400-886-0853 www.arexbio.com info@arexbio.com

  • Previous:
  • Next: