Biotin Anti-Human IgE Rabbit Monoclonal Antibody, Clone RM122

$362.00

Biotinylated Rabbit monoclonal to Human IgE

50 µg


  • Tags:31-1024-02, 31102402
  • SKU:31-1024-02
  • Categories:Biotin Conjugated Human Ig, Rabbit Monoclonal, Secondary Antibodies
RevMAb in China:

Description

Product Details
Product NameBiotin Anti-Human IgE Rabbit Monoclonal Antibody, Clone RM122
Product DescriptionBiotinylated Rabbit monoclonal to Human IgE
Catalog No.31-1024-02
Clone NameRM122
Product Data SheetBiotin Anti-Human IgE Rabbit Monoclonal Antibody Data Sheet RM122
SpecificityRM122 reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA.
ImmunogenHuman IgE
ApplicationELISA
Species ReactivityHuman
ConjugateBiotin
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size50 µg
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 10 ng/well – 100 ng/well (for Capture);
0.01 µg/mL – 0.1 µg/mL (for Detection);
Keywords
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Product Images
Sandwich

Sandwich ELISA using RM122 as the capture antibody
(25 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.

ELISA

ELISA of human immunoglobulins shows RM122 reacts
only to human IgEλ from human myeloma plasma
and the IgE from human plasma. No cross reactivity
with Human IgG, IgM, IgD, or IgA. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL or 10 ng/mL of RM122 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.

A

A titer ELISA using RM122. The plate was coated
with different amounts of human IgE.A serial dilution
of RM122 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.

RevMAb in China: AREX Biosciences Ltd. 400-886-0853 www.arexbio.com info@arexbio.com

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