Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120

$362.00

Biotinylated Rabbit monoclonal to Human IgG4

50 µg


  • Tags:31-1022-02, 31102202
  • SKU:31-1022-02
  • Categories:Biotin Conjugated Human Ig, Rabbit Monoclonal, Secondary Antibodies
RevMAb in China:

Description

Product Details
Product NameBiotin Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120
Product DescriptionBiotinylated Rabbit monoclonal to Human IgG4
Catalog No.31-1022-02
Clone NameRM120
Product Data SheetBiotin Anti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120
SpecificityRM120 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
ImmunogenPeptide corresponding to the hinge region of Human IgG4
ApplicationELISA
Western Blot
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
Flow Cytometry (FC)
Species ReactivityHuman
ConjugateBiotin
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size50 µg
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Western Blot (WB): 0.5 µg/mL – 2 µg/mL;
Immunocytochemistry (ICC): 1 µg/mL - 10 µg/mL;
Immunohistochemistry (IHC): 1 µg/mL - 10 µg/mL;
Flow Cytometry (FC): 1 µg/mL - 4 µg/mL.
Keywords
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Product Images
Sandwich




Sandwich ELISA using RM120 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ)antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.

Sandwich



Sandwich ELISA using RM120 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ)antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.

Western



Western Blot of human, mouse, rat, and goat
IgG shows RM120 reacts to human IgG4, in
both whole molecule (~150kDa, non-reduced)
and heavy chain (~50kDa, reduced) forms. No
cross reactivity with other isotypes of human IgG,
or mouse, rat, or goat IgG.

A



A titer ELISA using RM120. The plate was coated
with different amounts of human IgG4. A serial dilution
of RM120 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.

Immunohistochemistry




Immunohistochemistry of Human Lymphoid Tissue using
Anti-Human IgG4 antibody, Clone RM120.

RevMAb in China: AREX Biosciences Ltd. 400-886-0853 www.arexbio.com info@arexbio.com

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