Human IgE Matched Antibody Pair

Human IgE Matched Antibody Pair

$501.00

It is designed for the quantitative detection and measurement of human IgE in biological fluids such as serum and plasma.

100 µg/25 µg


  • Tags:31-1024-MK, 310124MK
  • SKU:31-1024-MK
  • Categories:Category: Matched Antibody Pair Kits
RevMAb in China:

Description

Product Details
Product NameHuman IgE Matched Antibody Pair [RM122/RM129]
Product DescriptionThe Human IgE Matched Antibody Pairs is specific to Human IgE and has been demonstrated through ELISA. It does not cross-react to IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), or mouse, rat or goat immunoglobulin. The pair can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data. This product includes capture and detection antibodies and sample diluent buffer.
Catalog No.31-1024-MK
Clone NameRM122 Anti-Human IgE Rabbit Monoclonal Antibody

RM129 Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody
Product Data SheetHuman IgE Matched Antibody Pair
SpecificityThe antibody pair detects only human IgE. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses.
ApplicationSandwich ELISA, or other Sandwich Assays
Species ReactivityHuman
ConjugateRM129: Biotin
Intended UseResearch Use Only
.ProtocolSandwich ELISA Protocol
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Components1)Capture Antibody: Anti-Human IgE Rabbit Monoclonal antibody, clone RM122, 100 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
2)Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
3)10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
Keywords
UsageELISA: Capture Antibody 50 ng/well – 200 ng/well;
Detection Antibody 0.1 µg/mL – 0.5 µg/mL
.
Product Images
Sandwich

Sandwich ELISA using RM122 as the capture
antibody (100 ng/well), and Biotinylated anti-human
light chains (k+l) antibody RM129 (0.2 µg/mL) as the
detection antibody, followed by an alkaline phosphatase
conjugated streptavidin.

.

Sandwich ELISA, using RevMAb human IgE matched
antibody pair (capture: RM122; detection: Biotin-RM129),
shows species reactivity to human only, and shows no
cross-reactivity to monkey (Cyno or Rhesus), mouse IgE,
rat IgE, or goat IgE.

Sandwich

Sandwich ELISA, using RevMAb human IgE matched antibody pair,
shows no cross- reactivity with Human IgG, IgM, IgA, or IgD.
The plate was coated with human IgE capture antibody (50ng/well).
Different immunoglobulin samples (0.2 µg/mL) were added and
biotinylated RM129 (0.2 µg/mL) was used as detection antibody,
followed by an alkaline phosphatase conjugated streptavidin.

RevMAb in China: AREX Biosciences Ltd. 400-886-0853 www.arexbio.com info@arexbio.com
  • Previous:
  • Next:
    • Hit enter to search or ESC to close