Anti-HA-Tag Chimeric Human Monoclonal Antibody, Clone RMH02

$386.00

Rabbit/Human Chimeric monoclonal to HA-Tag
containing Human IgG1 CH and Cκ

100 µg


  • Tags:54-1191-00, 54119100
  • SKU:54-1191-00
  • Categories:Epitope Tags, Primary Antibodies, Rabbit/Human Chimeric Antibody
RevMAb in China:

Description

Product Details
Product NameAnti-HA-Tag Rabbit/Human ChimericMonoclonal Antibody, Clone RMH02
Product DescriptionRabbit/Human Chimeric monoclonal to HA-Tag

This antibody is a Rabbit-Human Chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HA-Tag monoclonal antibody (RM305), with the constant domain of Human IgG1. This antibody is recognized only by an anti-Human IgG secondary antibody.
Catalog No.54-1191-00
Clone NameRMH02
Product Data SheetAnti-HA-Tag Rabbit/Human Chimeric Monoclonal Antibody Data Sheet RMH02
SpecificityRMH02 reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein.
ImmunogenA peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106).
ApplicationWestern Blot
ELISA (Capturer or Detector)
Species ReactivityAll
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 µg
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit/Human Chimeric
Rabbit Fv/ Human IgG1 CH and Cκ
UsageELISA: Capturer: 0. 5 µg/mL – 2 µg/mL;
Detector: 0.01 µg/mL – 0.5 µg/mL;
Western Blot: 0.1 µg/mL – 1 µg/mL.
Keywords
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Product Images
Sandwich

Sandwich ELISA performed on whole lysate of LNCaP
cells overexpressing HA-tag Bag1 protein. ELISA plate
was coated with 50 µL/well of chimeric human anti-HA-
Tag antibody Clone RMH02 (1 µg/mL) as the capture
antibody. Anti-Bag1 rabbit serum was used as the
detection antibody, followed by an alkaline phosphatase
conjugated anti-Rabbit IgG as the Secondary antibody.

Western

Western blot of 293T cells transfected (+) or non-
transfected (-) with a DNA construct encoding HA-
Tag KRas fusion protein, using anti-HA-tag rabbit
monoclonal antibody (clone RM305) at 0.1 µg/mL,
followed by a HRP conjugated anti-Human IgG
secondary antibody.

RevMAb in China: AREX Biosciences Ltd. 400-886-0853 www.arexbio.com info@arexbio.com

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