Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody, Clone RM307

$362.00 – $881.00

Rabbit monoclonal to Histone H3.3 G34V;
Histone H3.3 G34V Mutant

100 µL Small Size, 400 µL Large Size, 50 µL Biotin Conjugate
Request Quote for Bulk Sizes


  • Tags:31-1193-00, 31-1193-00-L, 31-1193-00-S, 31-1193-02, 31119300, 31119302
  • SKU:31-1193-00
  • Categories:Biotin Conjugates, Histone Mutation, Histone Mutations, IHC Primary Abs, Mutation Specific Antibodies, Primary Antibodies, Rabbit Monoclonal
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Description

Product Details
Product NameAnti-Histone H3.3 G34V Rabbit Monoclonal Antibody, Clone RM307
Product DescriptionRabbit monoclonal to Histone H3.3 G34V; Histone H3.3 G34V Mutant
Catalog No.31-1193-00-S;
31-1193-00-L;
31-1193-02
Clone NameRM307
Product Data SheetAnti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307

Biotinylated Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307
SpecificityRM307 reacts to the Histone H3.3 G34V mutant. No cross reactivity with wild type Histone H3.3 .
ImmunogenA peptide corresponding to Histone H3.3 G34V mutant
ApplicationWestern Blot
ELISA
Immunohistochemistry

Species ReactivityAll
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 µL;
400 µL;
Biotinylated 50 µL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 1:1000 – 1:10000;
ELISA: 1:500 – 1:5000;
IHC: 1:100 - 1:500.
References for Histone H3.3 G34V Mutant antibody [RM307]Nambiraja A et al C19MC amplification and expression of Lin28A and Olig2 in the classification of embryonal tumors of the central nervous system: A 14-year retrospective study from a tertiary care center. Childs Nerv Syst. (2021), 10.1007/s00381-020-04973-0. IHC; Human. Read more (PubMed: 33236184)

Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602)

Sangatsuda Y et al. Base-resolution methylomes of gliomas bearing histone H3.3 mutations reveal a G34 mutant-specific signature shared with bone tumors. Neuro Oncol. (2020), 10.1093/neuonc/noaa222.836. WB; Human. Read more (PubMed: PMC7715604)

Ito Y et al. Giant cell-rich osteosarcoma of the vertebra with murine double minute chromosome 2- and cyclin-dependent kinase 4-positive and histone H3F3A mutant p.Gly34Trp-negative immunophenotypes. Pathol Int. (2018), 10.1111/pin.12650. IHC; Human. Read more (PubMed: 29446514)

Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)
.
Product Images
Immunohistochemical

Immunohistochemical staining of Giant Cell Bone Tumor (GCBT)
tissue sections using Anti-Histone H3.3 G34V antibody, Clone RM307.


Image courtesy of Hidetaka Yamamoto, MD, PhD,
Kyushu University, Fukuoka, Japan

Immunohistochemical


Immunohistochemical staining of formalin fixed and
paraffin embedded 293T cells transfected with a
DNA construct encoding Histone H3.3 wild type
or G34V mutant, stained with anti-Histone H3.3
G34V rabbit monoclonal antibody, Clone RM307.

Western


Western Blot analysis of cell lysates prepared from
293T, transfected with a DNA construct encoding
wild type or G34V mutant proteins of Histone H3.3,
using anti-Histone H3.3 G34V rabbit monoclonal
antibody, Clone RM307.

RevMAb in China: AREX Biosciences Ltd. 400-886-0853 www.arexbio.com info@arexbio.com

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