Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119

$362.00

Rabbit monoclonal to Human IgG3

100 µg

Tags:31-1021-00, 31102100
SKU:31-1021-00
Categories:Anti-Human Ig, Primary Antibodies, Rabbit Monoclonal

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Description

Product Details
Product Name	Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119
Product Description	Rabbit monoclonal to Human IgG3
Catalog No.	31-1021-00
Clone Name	RM119
Product Data Sheet	Anti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119
Specificity	RM119 reacts to the heavy chain of human IgG3, RM119 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG4), and shows no cross reactivity IgM, IgA, IgD, IgE. RM119 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Immunogen	Human IgG3
Application	ELISA Immunocytochemistry (ICC) Immunohistochemistry (IHC)
Species Reactivity	Human
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	100 µg
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: 50 ng/well – 200 ng/well (for Capture); 0.05 µg/mL – 0.2 µg/mL (for Detection); Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL; Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL.
References for Human IgG3 antibody [RM119]	Sulka KB et al. Lyme Disease-Associated IgG4 Autoantibodies Correlate with Synovial Pathology in Antibiotic-Refractory Lyme Arthritis. Arthritis Rheumatol. (2018) 10.1002/art.40566. IHC-P; Human. Read more (PubMed: 29790305)
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Product Images
	Detection of human IgG3 in monkey serum. Sandwich ELISA using RM119 as the capture antibody, and biotinylated anti-human light chains ( κ+ λ)antibody, Clone RM129, as the detection antibody, followed by a HRP conjugated streptavidin.
	Sandwich ELISA using RM119 as the capture antibody (100 ng/well), and biotinylated anti-human light chains ( κ+ λ) antibody, Clone RM129, as the detection antibody, followed by an HRP conjugated streptavidin.
	ELISA showing RM119 reacts only to human IgG3, and not to any other IgG subclasses (IgG1, IgG2, or IgG4), and no cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
	A titer ELISA using RM119. The plate was coated with different amounts of human IgG3. A serial dilution of RM119 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
	Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section using anti-human IgG3 rabbit monoclonal antibody, Clone RM119.
	ELISA showing RM119 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM119 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
	Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG3 antibody, Clone RM119 at a 1:2000 dilution. Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA